anti β actin (Proteintech)
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Anti β Actin, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B2+actin/HNRNPA3+Antibody/pmc13019968-266-8-13
Average 93 stars, based on 24 article reviews
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Incubation:Article Title: Lung cancer cell-derived exosomal EHF drives M2 macrophage polarization via transcriptional activation of RNF41 to promote tumor progression Article Snippet: Protein samples were denatured with SDS loading buffer and boiled for 5 min, separated by SDS-PAGE, then transferred to PVDF membranes (Millipore, Billerica, MA, USA). .. Membranes were blocked with 5% skim milk in TBST, incubated with primary antibodies against EHF (1:1000, Catalog #: 27195-1-AP, Proteintech, Wuhan, China), TSG101 (1:1000, Catalog #: 14497-1-AP, Proteintech), Calnexin (1:1000, Catalog #: 10427-2-AP, Proteintech), CD9 (1:2000, Catalog #: 60232-1-Ig, Proteintech), RNF41 (1:500, Catalog #: 17233-1-AP, Proteintech), CD206 (1:1000, Catalog #: 18704-1-AP, Proteintech), TGF-β1 (1:1000, Catalog #: 26155-1-AP, Proteintech), VEGFA (1:1000, Catalog #: ab46154, Abcam, Cambridge, UK), and Article Title: SPARC upregulation mediates podocyte injury in Alport syndrome mice. Article Snippet: .. Membranes were incubated with primary antibodies: SPARC (1:1000, Proteintech, 15274-1-AP), ADGRB1 (1:1000, Abcam, ab135907), Podocin (1:1000, Abcam, ab50339), Nephrin (1:1000, ProSci, 2265), p-PI3K (1:2000, Cell Signaling Technology, 17366S), PI3K (1:2000, Cell Signaling Technology, 4292S), p-AKT (1:2000, Cell Signaling Technology, 4060S), AKT (1:2000, Proteintech, 10176-2- AP) and Article Title: Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progression Article Snippet: The membrane was then incubated with the MFSD2B primary antibody (Invitrogen, PA5-21050) overnight at 4°C. .. Afterward, the membrane was incubated with the secondary antibody (Abiowell, Article Title: A dual-responsive CO-releasing nanogel ameliorates retinal ischemia–reperfusion injury by restoring mitochondrial homeostasis and attenuating cGAS-STING pathway activation Article Snippet: .. The following primary antibodies were used: Drp1 (CST, #8570, 1:1000, ∼80 kDa), Fis1 (Proteintech, 10956-1-AP, 1:1000, ∼17 kDa), Mfn1 (Abcam, ab104274, 1:1000, ∼84 kDa), Mfn2 (CST, #9482, 1:1000, ∼86 kDa), Opa1 (CST, #80471, 1:1000, ∼100–120 kDa), Pink1 (CST, #6946, 1:1000, ∼63 kDa), cGAS (CST, #15102, 1:1000, ∼60 kDa), STING (CST, #13647, 1:1000, ∼42–45 kDa), TBK1 (CST, #3013, 1:1000, ∼84 kDa), p-TBK1 (CST, #5483, 1:1000, ∼84 kDa), COX IV (Abcam, ab14744, 1:2000, ∼17 kDa), Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and Membrane:Article Title: Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progression Article Snippet: The membrane was then incubated with the MFSD2B primary antibody (Invitrogen, PA5-21050) overnight at 4°C. .. Afterward, the membrane was incubated with the secondary antibody (Abiowell, Control:Article Title: Insufficient erythrocyte-derived S1P: A pathogenic driver and diagnostic biolipid for tumor progression Article Snippet: The membrane was then incubated with the MFSD2B primary antibody (Invitrogen, PA5-21050) overnight at 4°C. .. Afterward, the membrane was incubated with the secondary antibody (Abiowell, Sequencing:Article Title: Knockdown of CCT2 inhibits the malignant progression of hepatocellular carcinoma cells by impairing STAT3 activation Article Snippet: .. The primary antibodies were as follows: CCT2 (cat. no. 24896-1-AP), Blocking Assay:Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and Binding Assay:Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques Article Snippet: .. To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and |
